– NF-?B-IN-1, a 4-arylidene crucumin analogue, is a potent NF-?B signaling pathway inhibitor. NF-?B-IN-1 directly inhibits IKK to block NF-?B activation. NF-?B-IN-1 effectively inhibits the viability of lung cancer cells and attenuates the clonogenic activity of A549 cells.